The mobile phase is driven to pass through the stationary phase by both pressure and EOF and the sample is therefore separated due to the chromatographic partition and electrophoretic mobility. Therefore, ionic and neutral compounds can be separated at the same time.
As the exist of electroosmotic flow, there is no worry about the back pressure .Therefore, ultra small particles can be used to get higher efficiency, better peak shape and lager peak capacity. And longer column can be used for better separation. The elcetroosmotic flow helps improve the sample pattern and molecule diffusion in the column, contributing to a far better column efficiency than HPLC.Advantages:
- Separation of charged and non-charged components at the same time
- Ultra high efficiency with the use of very small particles
- Low consumption of solvent and sample
- Fast speed of separation
- pCEC, nano-LC and CE, all in one